Super-resolution imaging reveals details inside living cells
Researchers at EPFL in Switzerland have developed SPIFFI, a single-exposure super‑resolution fluorescence microscopy technique that uses polarized light to image dynamic cellular structures such as mitochondria and microtubules. SPIFFI achieves up to a two‑fold resolution improvement, enabling real‑time visualization of fast cellular processes and producing super‑resolved videos from each frame. The method can be integrated with existing microscopes and combined with post‑processing techniques to reach ~80‑nanometer resolution.
Source: EPFL News